Fig. 5.

Effects of nuclear receptor agonist treatment on metabolic gene expression in chondrocytes. IMACs were incubated for 72 h with 1 μM DMSO (vehicle control), PPARδ agonist GW501516, PPARγ agonist Rosiglitazone, LXR agonist GW3965, or RXR agonist SR11237. a, c, d, g, i Relative gene expression of Abca1, Cidea, Cpt1a, Lpl, and Insig1 is significantly increased by PPARδ, LXR, and RXR treatments. b, f Relative gene expression of Angptl4 and Pdk4 is elevated by all four treatments. e Relative gene expression of Fabp3 is significantly upregulated by PPARδ agonism only. g RXR and PPARδ treatment significantly decreased relative gene expression of Gsta4. h Srebf1 expression is significantly upregulated by LXR agonism only. Values represented are the mean ± SEM of ≥3 independent cell isolations. *p < 0.05