Fig. 5.

Membrane bound iOGN interacts with TLR4 on leukocytes. a Docking predictions of TLR4 and OGN reveal a potential interaction between these proteins via their leucine-rich repeats. b HEK-Blue™-mTLR cells containing an inducible SEAP reporter gene were stimulated with TLR-specific ligands (Pam3CSK4 for TLR1/2, Poly(I:C) (HMW) for TLR3, LPS-EK for TLR4, FLA-ST for TLR5, FLS-1 for TLR6/2, and ODN1826 for TLR9) with and without OGN knockdown. OGN knockdown reduced the activation of TLR3, -4 and -5. c, d OGN co-immunoprecipitated with TLR4 in human peripheral leukocytes and murine bone marrow-derived macrophages. e Confocal immunofluorescence revealing the co-localization of OGN with TLR4 in primary murine macrophages. n ≥ 4, *p < 0.001. All experiments were repeated at least twice. Scale bar 50 µm