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. 2016 Dec 21;292(2):365–383. doi: 10.1007/s00438-016-1277-x

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Cell migration, cell polarity and cell size analysis. a Analysis of speed during migration in µm/min (***P < 0.001). 32 cells each were analyzed. b Cell polarity analysis. Cells migrating into a scratch wound were fixed after 7 h of migration and stained for the Golgi (anti 58 K, mAb), centrosome (anti-pericentrin, pAb) and the nuclei (DAPI). The cells were migrating towards the lower right (location of the wound). The arrow points to a centrosome which is not colocalizing with the Golgi apparatus. Scale bar 10 µm. c Cell size of control and patient cells in micrometers. ~220 and 390 cells were analyzed, respectively