Figure 1. CPEs containing 3′-UTR confer in vitro oncoselectivity to engineered transgenes.

(a) The upper panel shows representative western blots showing CPEB1 and CPEB4 expression in pancreatic primary fibroblasts, normal cells (HPDE) and tumour cells (RWP-1, MIA PaCa-2 and PANC-1). The lower panel shows quantification of CPEB1 and CPEB4 signals normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (b) Schematic representation of the assessed 3′-UTR. Regulatory sequences are indicated. (c) Quantification of relative d2EGFP/dRFP fluorescence intensity levels in cell lines transduced with the indicated lentiviruses and relative to the mean intensity/content of the d2EGFP/dRFP from cells transduced with Lv-WT 3′-UTR. Data are shown as mean±s.e.m. from three independent biological replicates and were analysed by a linear mixed model fit by REML and a Tukey's contrast test to assess the significance of the differences. **P<0.01, ***P<0.001. cCPE, consensus CPE; ncPCE, nonconsensus CPE.