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. Author manuscript; available in PMC: 2017 Mar 18.
Published in final edited form as: Cell Rep. 2017 Feb 21;18(8):2058–2072. doi: 10.1016/j.celrep.2017.01.075

Figure 1. Diverse retinal cell types labeled by Cre-dependent rAAV reporter in Cre driver lines.

Figure 1

(a) Sketch of retina showing cell types and layers. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; R, rod photoreceptor; C, cone photoreceptor; H, horizontal cell; B, bipolar cell; A, amacrine cell; M, Muller glia; RGC, retinal ganglion cell. (b–i) Cre-expressing lines were injected intravitreally with rAAV encoding Cre-dependent EGFP. Sections were stained with anti-GFP. Section in (b) was also stained with DAPI to mark all cells. Section in (c) was stained with anti-AP2a (red) to mark amacrine cells and anti-VAChT (blue) to mark dendrites of starburst amacrine cells in the IPL. Sections in (h,i) were labeled with anti-RBPMS and anti-VAChT to mark both RGCs and dendrites of starburst amacrine cells; arrowheads point to RGCs. In (a–g) bracket marks IPL layer. (b) RGCs and amacrines in Grm2-Cre_MR90. (c) RGCs in Slc17a6-IRES-Cre. (d) RGCs, amacrines and horizontal cells in Cux2-IRES-Cre. (e) Narrow-field amacrine cells in Pnmt-Cre. (f) A17 amacrine cell in Slc6a5-Cre_KF109. (g) RGCs and amacrine cells in Wfs1-Tg3-CreERT2. Pcdh9-Cre_NP276 (e) and Grik4-Cre (f) selectively label RGCs that cofasciculate with starburst amacrine cells in S4, a characteristic of ON-DSGCs. Grik4-Cre also contains some CART-positive ooDSGCs (not shown). Scale bars 25μm.