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. 2017 Mar 18;18:176. doi: 10.1186/s12859-017-1591-2

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Example data sets ordered with respect to increasing segmentation complexity a) micrograph showing chlorophyll inside chloroplasts after Fluorescein diacetate (FDA) staining; b) Multi channel data set of naive murine B cells from C57Bl/6 mice B cells. Immunofluorescent staining was performed for DNA (DAPI); c) Immunofluorescent staining was performed for F-Actin (Phalloidin-Rhodamin); d) LPS activated macrophages with immunofluorescent staining CD11b/APC; Scale bars corresponds to 30 μm