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. 2016 Oct 7;42(3):777–787. doi: 10.1007/s11064-016-2075-x

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — TBBPA-induced increases in intracellular Ca²⁺ concentration in primary CGC cultures. a The concentration-dependent effects of TBBPA versus vehicle (0.5 % DMSO) on the fluorescence of fluo-3. b Modulation of the effects of 10 and 25 µM TBBPA by the free radical scavengers 10 µM butylated hydroxyanisole (BHA) dissolved in 0.1 ‰ ethanol (ET), 1 mM reduced glutathione (GSH), 1 mM ascorbic acid (vit. C), and the combination of RyR and NMDAR antagonists 2.5 µM bastadin 12 (bast), 200 µM ryanodine (ryan) and 0.5 µM MK-801 (MK). Fluorescence of fluo-3 is expressed as percentage of the basal level (∆F/F₀ %) (a), or percent of control at 30 min (b). The results are the mean values ± SD (n = 15). *Results significantly different from the control. ^#Results significantly different from the corresponding group treated only with TBBPA (p < 0.05)