Fig. 2.

Increase in production of reactive oxygen species in primary cultures of CGC treated with TBBPA. a The concentration-dependent effects of TBBPA versus vehicle (0.5 % DMSO) on the fluorescence of DCF. b Modulation of the effects of 10 and 25 µM TBBPA by the free radical scavengers 1 mM reduced glutathione (GSH), 10 µM butylated hydroxyanisole (BHA) dissolved in 0.1 ‰ ethanol (ET), MPTP inhibitor 5 µM cyclosporin A (CsA), and the combination of RyR and NMDAR antagonists 2.5 µM bastadin 12 (bast), 200 µM ryanodine (ryan) and 0.5 µM MK-801 (MK). Fluorescence of DCF is expressed as percentage of the basal level (∆F/F₀ %) (a), or percent of control at 30 min (b). The results are the mean values ± SD (n = 15). *Results significantly different from the control. ^#Results significantly different from the corresponding group treated only with TBBPA (p < 0.05)