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. 2016 Dec 27;40(2):497–510. doi: 10.1007/s10753-016-0495-z

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 6 — Ephx2^−/− mice had less autophagy in response to CS-WT and Ephx2^−/− mice were exposed to air or CS for 12 weeks and autophagy protein LC3B and Beclin 1 expression was measured. a Autophagosomes under electron microscopy (×100,000); white arrows depict autophagosomes (n = 3 mice/group). b EM images scored autophagosomes. Data are expressed as AVs/100 μm² (N = 10 images representative of each group). c Western blot of Beclin 1. d Relative intensity of Beclin 1 was calculated by densitometry and values are presented as means ± SD. e–h Representative immunohistochemical images for Beclin 1 (e) and LC3B (g) in mouse lung tissues (n = 3 mice/group). f, h Graph represents IntDen of Beclin 1 and LC3B and values are presented as means ± SD. ^# P < 0.05, significant difference from corresponding air-exposed mice; ^## P < 0.01, significant difference from corresponding air-exposed mice. ^* P < 0.05, significant difference between CS-exposed WT mice and CS-exposed Ephx2^−/− mice.

Fig. 6 — Ephx2^−/− mice had less autophagy in response to CS-WT and Ephx2^−/− mice were exposed to air or CS for 12 weeks and autophagy protein LC3B and Beclin 1 expression was measured. a Autophagosomes under electron microscopy (×100,000); white arrows depict autophagosomes (n = 3 mice/group). b EM images scored autophagosomes. Data are expressed as AVs/100 μm² (N = 10 images representative of each group). c Western blot of Beclin 1. d Relative intensity of Beclin 1 was calculated by densitometry and values are presented as means ± SD. e–h Representative immunohistochemical images for Beclin 1 (e) and LC3B (g) in mouse lung tissues (n = 3 mice/group). f, h Graph represents IntDen of Beclin 1 and LC3B and values are presented as means ± SD. ^# P < 0.05, significant difference from corresponding air-exposed mice; ^## P < 0.01, significant difference from corresponding air-exposed mice. ^* P < 0.05, significant difference between CS-exposed WT mice and CS-exposed Ephx2^−/− mice.