Table 1.
Characteristics of the specific primers used for real-time RT-PCR analysis.
| Gene | Protein | Species | Sequence | bp | NCBI reference |
|---|---|---|---|---|---|
| Actb | Beta-actin | Human | Fw: GACGACATGGAGAAAATCTG | 131 | NM_001101 |
| Rv: ATGATCTGGGTCATCTTCTC | |||||
| Ppia | CypA | Rat | Fw: GTGTTCTTCGACATCACG | 92 | NM_017101 |
| Rv: TGTCTGCAAACAGCTCGAAG | |||||
| Klrk1 | NKG2D | Rat | Fw: TTAATGAGAACAAAGCCTGG | 199 | NM_133512 |
| Rv: GTTAACTCGTTGGGTGATAG | |||||
| Klrk1 | NKG2D | Human | Fw: AGGACAAAATGACCAAAGAC | 135 | NM_007360 |
| Rv: CTTGGGGATATCTGAATTGC | |||||
| Ncr1 | NKp46 | Rat | Fw: AGACCCTGTTTCTTCTCTTG | 197 | NM_057199 |
| Rv: TGAGCTTCTCATGATCCTTC | |||||
| Ncr1 | NKp46 | Human | Fv: CAGAAATGTATGACACACCC | 119 | NM_001145457 |
| Rv: CTTGAGCAGTAAGAACATGC | |||||
| Ncr3 | NKp30 | Rat | Fw: AAGAGCCTCCTCAACAAG | 94 | NM_181822 |
| Rv: AGAAAGCTGAGGGCATAG | |||||
| Ncr3 | NKp30 | Human | Fw: ATATGCCAAATCTACTCTCTCC | 200 | NM_001145466 |
| Rv: TCTGATCCTTCCCATTTCTC |