Table 4.

Preparation of E8 medium

Component	Stock solution	Take from stock	Final concentration
DMEM/F12 with glutamine and HEPES	1×	500 ml	1×
Sodium bicarbonate	75 mg/ml	3.62 ml	543 μg/ml
L-Ascorbic acid 2-phosphate	64 mg/ml	500 μl	64 μg/ml
Insulin	4 mg/ml	2.5 μl	20 μg/ml
Transferrin	53.5 mg/ml	100 μl	10.7 μg/ml
Sodium selenite	700 μg/ml	10 μl	14 ng/ml
FGF2	100 μg/ml	500 μl	100 ng/ml
TGFβ	100 μg/ml	10 μl	2 ng/ml
Pen/strep	100×	5 ml	1×