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. 2017 Mar 20;8:460. doi: 10.3389/fmicb.2017.00460

FIGURE 1.

FIGURE 1

Inhibition of SLY-induced hemolysis by morin. (A) The chemical structure of morin. (B) The inhibitor effect of morin on the hemolytic activity of bacterial culture supernatants after co-culture with SS2. SS2 was co-cultured with morin, and the hemolytic activity of culture supernatants was determined using a hemolysis assay. The percent hemolysis of each sample was calculated by comparison with the control sample. (C) Western blot analysis of the SLY level in the culture supernatants. (D) The growth of SS2 in the presence of the indicated morin concentrations. SS2 was co-cultured with various concentrations of morin, and the growth kinetics were determined by measuring the OD600 of each sample every 30 min. (E) Inhibition of purified rSLY activity by morin. Following an incubation with the indicated concentrations of morin, the hemolytic activity of rSLY was examined by hemolysis assay. ∗ indicates P < 0.05, and ∗∗ indicates P < 0.01 compared with the morin-free sample (two-tailed Student’s t-test).