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. 2017 Mar 20;8:139. doi: 10.3389/fphar.2017.00139

FIGURE 4.

FIGURE 4

Chronic (6 months daily) intraperitoneal injection of ketamine at 30 and 60 mg/kg increased the protein level and the immunostaining level of IL-6. (A) Six months intraperitoneal injection of ketamine increased the protein level of IL-6. (B) Quantification of Western blot shows that 6 months intraperitoneal injection of ketamine significantly increased IL-6 level normalized to β-actin as compared with saline-treated group. (C) Quantification of the immunohistochemistry image shows that the ketamine administration at the 60 mg/kg significantly increased the IL-6 immunostaining level in mouse hippocampus. (D–F) Immunohistochemistry studies show that the ketamine administration increased the IL-6 immunostaining level in mouse hippocampus. Scale bar = 100 μm. Data are expressed as mean ± SD, n = 10 of each group for proteomic analysis and n = 6 of each group for immunohistochemical staining. Statistical analysis used ANOVA to compare the difference between saline-treated group and each ketamine-treated group (*p < 0.05).