Figure 3.

D4 reverses the effect of inducible LeNCED1 expression on radicle emergence in transgenic tobacco seed. (A) Tobacco seed were imbibed in the absence of tetracycline on filter paper wetted with 0–50 μM ABA and mean germination time was calculated; the curve was fitted using a three parameter exponential rise to maximum model (R² = 0.95; p < 0.001). (B) Tobacco seed were sown on filter paper wetted with or without 10 mg l⁻¹ tetracycline and 1 mM D4, as indicated; all treatments additionally contained 0.2% DMSO, required to solubilize D4; ^*indicates significant difference (p < 0.05) compared to 0.2% DMSO control, according to ANOVA least significant difference; in both (A,B), mean germination time is based on radicle emergence. (C,D) show a time course of radicle emergence and hypocotyl emergence, respectively, with or without 10 mg l⁻¹ tetracycline (Tet) and 1 mM D4, as indicated. Error bars indicate standard error (n = 3); all treatments also contained 0.2% DMSO.