Figure 6.

The effect of D4 on seedling vigor in tomato and tobacco overexpressing LeNCED1. (A) LeNCED1-overexpressing tomato seed (sp12) were germinated on filter paper containing 0.1% DMSO with or without 1 mM D4, as indicated, and were transferred to soil following germination. Twenty shoots were harvested for measurement of dry mass, fresh mass, and length for each treatment at 5, 13, and 20 days post-germination. Error bars indicate standard error (n = 20). (B) Tobacco seed harboring tetracycline-inducible LeNCED1 were sown on 0.6% agar containing ½ MS salts and 0–200 μg l⁻¹ tetracycline with and without 1 mM D4 in 0.2% DMSO. Seed were incubated at 25°C in darkness for 6 days and then transferred to light conditions. Seedlings were photographed 10 days after sowing and green leaf area of seedlings was quantified using ImageJ. Error bars indicate standard error (n = 5). (C) Images of seedlings in plate wells representative of labeled treatments.