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. 2017 Mar 20;7:44687. doi: 10.1038/srep44687

Figure 5. siRNA-mediated knockdown of HSP70-1 led to the dysfunction of IL-5-induced proliferation, migration, colony tube formation, and phosphorylation of eNOS in HUVECs.

Figure 5

(a–e) IL-5-induced proliferation, migration, invasion, and colony tube formation was determined in HSP70-1 siRNA (si-HSP70-1-#1) transfected HUVECs. (f) After transfection of HSP70-1 siRNA (si-HSP70-1-#1) or scrambled siRNA for 24 h, cells were subjected to immunoblot analysis using specific antibodies for ERK1/2, AKT, and eNOS (S1177). (g) Serum-starved HUVECs were incubated with IL-5 for indicated times. Equal amounts of cell lysates were immunoprecipitated with anti-HSP70 antibody and analyzed by immunoblot using antibodies specific for eNOS and actin. All data are reported as the means ± SE from three independent experiments. *P < 0.05 compared with control, #P < 0.05 compared with IL-5 treatment.