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. 2016 Dec 6;4(23):e13048. doi: 10.14814/phy2.13048

Figure 8.

Figure 8

Alternating T‐maze experimental design and behavioral results. (A) Experimental design: There are two phases of T‐maze training on Day 1 and Day 2, where animals tended to make significantly more correct choices on Day 2. Each phase is composed of 30 trials of randomly ordered 6‐Hz yellow control, and blue light stimulations at 6 Hz or 30 Hz; (B) For testing, the rat is placed in the start area of the T‐maze. The gate is opened and the rat runs to end of the choice alley. For the first run, either the right or left arm is closed and the rat goes into the open arm with the food award. On the second trial, both arms are open and the rat is rewarded if it goes into the previously unbaited arm. This sequence is repeated for 30 test trials; (C) Statistical analysis demonstrated a significant increase in the percentage of correct choices during both 6‐Hz and 30‐Hz blue light stimulation on Day 1 of T‐maze training in comparison with 6‐Hz yellow light control stimulation. Performance on Day 2 across stimulation types did not differ significantly. Performance during control trials improved significantly between Day 1 and Day 2. (* = statistical significance).