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. 2016 Dec 6;4(23):e13056. doi: 10.14814/phy2.13056

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Tgfbr1 ^iko femoral arteries (FAs) enclose more inflammatory infiltrates than do Tgfbr1 ^f/f FAs at the advanced stage. (A) Immunofluorescence staining of CD45 in 28‐day‐old FA lesions. An uninjured FA was included as a biological negative control (i.e., no CD45 signal in the tunica media) in the assays. A panel of “no primary antibody” negative control is shown on the far right. Red: CD45⁺ cells (arrowheads); green: autofluorescence; white dashed lines: EEL. Scale bar, 50 μm. Fraction of CD45⁺ area in the myointima (B) and adventitia (C) 28 days after injury (n = 7 per genotype). P > 0.05 (B), **P = 0.009 (C), t‐test.