Figure 5. PML-RARα supresses NF-κB activity.
WT MEFs were transfected with empty vector (control) or PML-RARα plasmid and were (A) treated with TNFα (10 ng/ml) for the indicated time prior to QPCR analysis for IL-6, ICAM1, CXCL2 and WNT11; (B) treated with TNFα (10 ng/ml) for 10 minutes prior to analysis by immunoblotting with the indicated antibodies.