Figure 2. Kidney-specific gene transfer through different injection routes, volumes, and DNA doses.

(a) Mice were injected in the renal pelvis or renal vein of the left kidney as indicated with 10 μg of pT-effLuc-Thy1.1 luciferase transposon and imaged the following day to detect luciferase expression. In (b) and (c), the photons/second emitted from the left kidney were quantified for various injection conditions. (b) Mice were injected in the renal pelvis with varying volumes of injection fluid while the transposon amount was held constant at 10 μg. (c) Mice were given varying doses of DNA in 100 μl volume (n = 3).