Figure 3. Renal pelvis injection reaches both medulla and cortex result in punctate areas of gene expression.

(a) Uninjected kidney. (b) QR buffer-injected kidney. (c) Injection of latex fluorescent microspheres in a 100 μl volume of QR buffer. Two fields of view were assembled to display the entire kidney section. (d) IVIS image showing gross areas of punctate gene expression in bisected kidneys six days following DNA injection of 10 μg of luciferase transposon into the renal pelvis. Shown are both bisected kidneys from two animals: the left kidney was injected with DNA while the right kidney was uninjected. Each kidney half is arranged with the renal pelvis pointing inward toward the other half and the cortex on the exterior.