Figure 8. Cyclophosphamide treatment for immunosuppression resulted in increased luciferase expression.

Mice were given either saline (grey dashes, n = 3) or four cyclosphosphamide treatments (black diamonds, n = 4) during the week of the renal pelvis injection. Lightning bolts on the x-axis represent each cyclophosphamide injection. Each mouse received a renal pelvis injection of 10 μg of hyperactive piggyBac plasmid with 50 μg of enhanced firefly luciferase transposon, each expressed from the EF-1α promoter. Radiance was determined by live animal imaging on the IVIS machine at the days indicated following the renal pelvis injection. Bracket indicates comparison of the areas under the curve. *p < 0.05, **p < 0.005 by Student’s one-tailed t-test assuming unequal variances.