Table 2. Substrate Scope of wt-CalB and Ser105Ala catalyzed BV-oxidation.
Entry | Substrate | Product | wt-CalB |
Ser105Ala |
||
---|---|---|---|---|---|---|
Conversion (%) | Selectivity (%) | Conversion (%) | Selectivity (%) | |||
1 | 99 | 93 | 99 | 100 | ||
2 | 95 | 48 | 51 | 97 | ||
3 | 92 | 46 | 47 | 99 | ||
4 | 79 | 35 | 38 | 96 |
General conditions: ketone (1 mmol), 30% aq. H2O2 (2 mmol), octanoic acid (1 mmol), 5 mg CalB (wild-type or S105A), ChCl/sorbitol (1.2 g), H2O (0.3 mL). T = 40 °C, time 48 h.