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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Neurobiol Aging. 2016 Nov 15;51:43–53. doi: 10.1016/j.neurobiolaging.2016.11.001

Figure 5. FYN 3’UTR haplotype functional analysis relative to FYN promoter haplotypes in human glial cell lines.

Figure 5

FYN 3’UTR activity was evaluated using luciferase reporter vectors (pGL4.10[luc2]) with both the FYN 3’UTR and FYN promoter haplotypes inserted as depicted in panels A-C. Promoter 1 plus 3’UTR reporter constructs consisted of FynB promoter region haplotype and the FYN 3’UTR (A and D, G, J). Promoter 2 plus 3’UTR reporter constructs consisted of FynΔ7 promoter region haplotypes (B and E, H, K). Promoter 3 plus 3’UTR reporter constructs consisted of FynT promoter region haplotypes (C and F, I, L). Promoter region haplotype reporter constructs and Promoter plus 3’UTR reporter constructs were transiently transfected into a human astroglial cell lines (U87, U138, U118) (DΔF). Results are presented as Firefly/Renilla luciferase activity for the promoter plus 3’UTR constructs relative to the promoter only constructs (Relative Quantification) and SEM for each construct, n = 6 for each group. Black lines denote a significant difference between reporter construct haplotypes (p-value of < 0.05 after Bonferroni correction for multiple comparisons).