Fig. 6.

PHA767491 suppresses HSV-2 IE gene expression, viral replication and HSV-2 induced necrosis. a L929 cells were pretreated with DMSO or PHA767491 at the indicated concentrations for 1h and infected with HSV-2 (MOI = 2) for 10h, the cell lysates were collected and subjected to western blot analysis. b L929 cells were pretreated with DMSO or PHA767491 for 1h and then infected with HSV-2 (MOI = 2) for 2h. The expression of indicated proteins were measured by quantitative PCR. c L929 cells were pretreated with DMSO or PHA767491 for 1h and infected with HSV-2 (MOI = 2) for 36h. The cell viability was determined by measuring ATP level