Figure 2.

Capillary Surfactometry. Box and whisker plots for capillary surfactometry results from both raw (blue) and chloroform/methanol extracted (red) BAL fluid and patency time (%) over 2 min – log scale. In most circumstances the raw BAL fluid has minimal surface activity – low capillary patency. The one animal with a high raw value was from the BVV group. This patency markedly improved with extraction suggesting reactivation of surfactant with removal of BAL oedema fluid, proteins, including cytokines and cellular debris. In 4 animals there was essentially no change in function with extraction. There were 2 animals in the CMV group, and one each in the BVV and CMV-RM in this population. No statistically different behaviour in surfactant function was seen between groups, either for raw or extracted surfactant function.