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. Author manuscript; available in PMC: 2018 Mar 7.
Published in final edited form as: Cell Metab. 2017 Feb 16;25(3):622–634. doi: 10.1016/j.cmet.2017.01.009

Figure 3. Expression of β-cell genes in murine α-cells lacking Dnmt1 and Arx.

Figure 3

(a) Schematic showing experimental design for Dox treatment of knock out and control animals.

(b–i) Immunostaining showing expression of α, β, and δ-cell markers Ins, Gcg, MafB, Nkx6.1, Sst, Pdx1, Slc2a2 and MafA with YFP in αiADKO mice 12 weeks after Dox treatment. Yellow boxes show specific area of islet enlarged and represented by arrows on the right to demonstrate gene expression within specific cells or sets of cells. Scale bars represent 25 μm.

(j–k) Quantification of α-to-β-cell conversion in αiADKO mice at the end of a 4 week chase and 12 week chase.

(l) Quantification of Ki67+ YFP+ cells at the end of a 4 week chase and 12 week chase compared to controls (N=3 mice). Bar graph data are represented as mean ± S.D. Dox, Doxycycline. N=4 mice per time point.