Figure 8.
cAMP inhibition in the presence of 1 nM to 10 μM 2-AG, AEA, or CP55,940. cAMP levels were measured in HEK-CRE cells 4 h after treatment with 10 μM forskolin (F) + 2-AG (A), AEA (B), or CP55,940 (C) ± 1 μM GAT100, Org27569, or PSNCBAM-1. (D) cAMP levels were measured in HEK-CRE cells 4 h after treatment with 10 μM forskolin (F) + 1 nM to 10 μM GAT100, Org27569, or PSNCBAM-1 alone. Concentration–response curves were fit using the allosteric modulator shift nonlinear regression models. N = 4.