Fig. 2.

Advantages of engineered organoids for studying the stem cell niche. (A) Constructing organoids from purified cellular components allows the direct measurement of input cell properties and labeling of different input populations. (B) In addition to the advantages of classical organoid models (outlined in Fig. 1), controlled organoid engineering provides tight experimental control over the numbers and types of cells in the resulting tissue. (C) Engineered organoids can incorporate non-cellular material, such as sensors that dynamically measure properties such as mechanical forces and signaling pathway activation within live tissues.