Fig. 2.

The lack of RB produces enlarged organoids and promotes S entry. (A) Representative wild-type and KO organoids at 15 and 28 DIV sectioned and immunostained against Ki67 and Sox2 and counterstained with DAPI. The graphs show the percentage of Ki67⁺/DAPI cells and the percentage of Ki67⁺/Sox2⁺ cells at both ages. (B) Plots on the left show representative wild-type and KO organoid cell cycle profiles at 15 and 28 DIV and the graphs on the right show the percentage of cells in each cell cycle phases at both time points. (C) Representative wild-type and KO organoids at 15 and 28 DIV sectioned and immunostained against BrdU and DCX, and counterstained with DAPI. The graphs show the percentage of BrdU⁺/DAPI cells and the percentage of BrdU⁺/DCX⁺ cells at both ages. The results are mean±s.e.m. of three to seven organoids from three independent experiments (*P<0.05, Student's t-test; n.s., not significant). Scale bars: 50 µm.