Table IV.

Sensitivity to dinitroanilines of and amino acid present at specific positions in α-tubulins from 10 eukaryotic organisms

Species	Class	Range of Dinitroaniline IC50	Amino Acid Present at Position							References for IC50 values
			16	24	136	239	252	253	268
Green foxtail	Monocotyledon	0.02–0.2 μm	Val	Tyr	Leu	Thr	Val	Asn	Met	Waldin et al. (1992); This work
Goosegrass	Monocotyledon	0.02–0.2 μm	Val	Tyr	Leu	Thr	Val	Asn	Met	Waldin et al. (1992)
Tobacco	Dicotyledon	0.9–1.0 μm	Val	Tyr	Leu	Thr	Val	Asn	Met	Anthony et al. (1999)
Carrot	Dicotyledon	>10 μm	Ile	Tyr	Leu	Thr	Val	Thr	Met	Vaughan and Vaughn (1988)
C. reinhardtii	Alga	1.0–18.5 μm	Val	Tyr	Leu	Thr	Ile	Thr	Met	Schibler and Huang (1991),James et al. (1993)
T. gondii	Protozoan	>0.5–<2.5	Ile	Phe	Leu	Thr	Val	Thr	Met	Morrissette et al. (2004)
T. thermophila	Protozoan	<7.5 μm	Val	Phe	Leu	Thr	Ile	Thr	Met	Stargell et al. (1992)
L. major	Protozoan	4.0 μm	Val	Phe	Met	Thr	Leu	Thr	Val	Chan et al. (1993)
T. brucei	Protozoan	6.0–7.0 μm	Val	Phe	Leu	Thr	Leu	Thr	Val	Chan et al. (1993)
Rat	Mammal	Not sensitive	Ile	Tyr	Leu	Thr	Leu	Thr	Pro	Morejohn et al. (1987)

IC₅₀ values are based upon whole-organisms bioassays using wild-type lines or populations (cell-based bioassay for rat), and correspond to dinitroaniline concentrations inhibiting 50% organism growth. Because no sequence from Tetrahymena thermophila is available, a sequence from the closely related species Tetrahymena pyriformis was used for comparison.