Skip to main content
. 2004 Dec;136(4):3968–3978. doi: 10.1104/pp.104.049411

Figure 5.

Figure 5.

Expression of GFP-fABD2 reveals a dynamic framework of AFs. A, Recovery from LatB treatment in washed N. tabacum protoplast populations; rapid recovery of filamentous actin within 1.5 h of washing. Insets show half-cell (approximately 15 μm), confocal projections of protoplasts treated with 1 μm LatB or 0.1% DMSO for 24 h. B, Recovery from LatB treatment in washed Arabidopsis leaf epidermis; 8 h of LatB treatment (a) caused near complete depolymerization of the network, contrasting normal arrays in 0.1% DMSO-treated controls (b). Substantial recovery of the actin network 1.5 h after LatB washout in GFP-fABD2-expressing plants (c) but not in plants expressing GFP-mTalin (d). C, Fluorescence recovery along bleached AF bundles occurs rapidly and in a pattern resembling the prebleach image; images show typical fluorescence recovery in bleached bundle over 45 s, with left, center, and right boxes used in recovery analysis shown. Fluorescence recovers to 84% of prebleach intensity by 45 s in this example. D, Detailed analysis of fluorescence recovery following bleaching of bundled AFs; graph shows recovery ratio for one end compared with the center; arrows on graph correspond to time point for images displayed on right (n = 7). E, Plotted mean fluorescence intensity over time along bleach window in a GFP-fABD2-tagged transvacuolar actin bundle. F, Plotted mean fluorescence intensity over time along bleach window in an FDA-stained transvacuolar strand; fluorescence recovery is uniform over the bleach window with FDA, but is irregular and occurs fastest at the edges of the bleach window in GFP-fABD2-expressing cells. Insets in A are confocal projections (half-cell) and images in B are single-plane images with a z-depth of 2.5 μm. Scale bar = 10 μm (A–C).