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. 2004 Dec;136(4):3968–3978. doi: 10.1104/pp.104.049411

Table III.

Rate constants and half-times for FRAP

Label Cell Type k t1/2
s−1 s
YFP Hypocotyl 0.16 ± 0.03 6.3 ± 0.8
FDA Hypocotyl 0.15 ± 0.021 5.9 ± 1.1
GFP-mTalin Hypocotyl 0.05 ± 0.005 13.4 ± 1.2
GFP-fABD2 Hypocotyl 0.05 ± 0.006 13.4 ± 1.0
Root hair 0.05 ± 0.007 16.1 ± 3.0
Guard cell 0.05 ± 0.003 14.7 ± 0.9
Leaf epidermal 0.06 ± 0.008 13.2 ± 1.3

Arabidopsis cells expressing GFP-fABD2, GFP-mTalin, or YFP, or nonexpressing cells labeled with FDA were photobleached, the recovery plotted, and an exponential function fitted. The k and t1/2 were derived from the exponential function. Values are means ± se, n ≥ 12.