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. 2004 Dec;136(4):3999–4009. doi: 10.1104/pp.104.051466

Figure 3.

Figure 3.

Virus-induced gene silencing of GFP transgene and suppression of viral replication in Arabidopsis and N. benthamiana. a, VIGS in the shoot meristem of Arabidopsis N9135 line. Control GFP expression (subsection i), VIGS after TRV-2b-GFPVIGS vector infection (subsection ii; M, shoot meristem region). b, VIGS of GFP transgene in roots of Arabidopsis. Nonsilenced control plants GFP expression (subsections i–iii). Roots exhibiting silencing of the GFP (subsections iv–vi). Arrow indicates positions of root meristem (B, subsection iv). c, Virus replication suppression in N. benthamiana roots. Plants inoculated with TRV-2b-DsRED and in the background 35S∷GFP expression, 5–8 dpi (subsection i). Note the reduced level of viral replication in the new root tissue produced after the virus has invaded the meristem. Root meristem at 13 dpi with detectable viral DsRED fluorescence indicating active viral replication and recovery (subsection ii; M, meristem; RC, root cap). Arrow A indicates the zone of constriction due to TRV invasion; arrow B indicates new root growth exhibiting suppression of viral replication. d, Relative number of plants exhibiting silencing of GFP transgene observed on whole Arabidopsis plants infected with TRV-Δ2b-GFPVIGS (n = 17) or TRV-2b-GFPVIGS (n = 23) vectors. Images in subsection iii and subsection vi are similar to subsection ii and subsection v, respectively, from underneath petri dish to visualize all roots.