Fig. 2.

Src activity is necessary for increased invadopodia formation after RhoG KD. Western blot analysis shows increased levels of phospho-Src (Src p418) (A) and phospho-FAK (FAK p397) (B) in RhoG-depleted cells. Data are mean±s.e.m. of three independent experiments. (C–F) CTRL and RhoG KD cells were cultured in the presence of vehicle control, 5 μM PP2 (C,D) or 5 μM PF-573228 (E,F) for 30 min followed by a 30 min incubation with PDBu. The efficiency of the inhibitors was tested by immunoblotting for phospho-Src (Src p418) (C) or phospho-FAK (FAK p397) (E). Quantifications in D and F show the percentage of cells with invadopodia, as determined by cortactin and actin staining. Data are mean±s.e.m. of three independent experiments. *P<0.05; **P<0.01; ***P<0.001; ns, not significant.