Table I.
Ratio
|
Mean Ratio ± s.e.m.
|
||||
---|---|---|---|---|---|
Control
|
COMT
|
||||
WT | 35S 17B | 35S 21B | ASB 2B | ASB 10B | |
FA/SA | 0.28 ± 0.06 | 0.35 ± 0.05 | 0.26 ± 0.06 | 0.42 ± 0.10 | 0.29 ± 0.06 |
FA/Cal | 1.11 ± 0.30 | 1.40 ± 0.38 | 1.21 ± 0.64 | 1.49 ± 0.54 | 0.82 ± 0.23 |
Cal/SA | 0.27 ± 0.06 | 0.30 ± 0.08 | 0.30 ± 0.07 | 0.50 ± 0.27 | 0.37 ± 0.07 |
FA/Sal | 0.18 ± 0.05 | 0.30 ± 0.07 | 0.24 ± 0.11 | 1.18 ± 0.25* | 0.72 ± 0.21* |
SA/Sal | 0.67 ± 0.11 | 0.90 ± 0.18 | 0.80 ± 0.19 | 3.47 ± 1.11* | 2.66 ± 0.56* |
Cal/Sal | 0.16 ± 0.01 | 0.23 ± 0.03 | 0.21 ± 0.03 | 0.96 ± 0.18* | 0.91 ± 0.18* |
The mean ratios of the LC-MS selected ion currents obtained for ferulic acid (FA), sinapic acid (SA), coniferaldehyde (Cal), and sinapaldehyde (Sal) are given for the control (wild-type and transgenic lines 35S 17B and 35S 21B) and COMT-deficient poplars (transgenic lines ASB 2B and ASB 10B). Significantly changed ratios, obtained by applying the nested ANOVA Model A, between both groups of poplar lines are indicated with an asterisk. s.e.m., se of the mean.