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. 2017 Mar 6;114(11):2952–2957. doi: 10.1073/pnas.1615601114

Fig. 2.

Fig. 2.

MS402, a bromodomain inhibitor, renders Th17 cell differentiation. (A) (Upper) A schematic illustration of the T-helper-cell differentiation study. (Lower) Flow cytometry analysis of mouse primary naïve CD4+ T cells purified from spleens and lymph nodes of C57BL/6 mice and differentiated under Th0, Th1, Th2, Th17, and Treg polarization conditions with and without the presence of MS402 added daily at 100 nM or 500 nM. (B) Table summarizing the effects of MS402 or pan-BET BrD inhibitors including MS417, JQ1, and I-BET762 on T-helper-cell differentiation. (C) Effects of MS402 or MS417 treatment on mRNA expression levels of key Th17, Th1, Th2, or Treg subset-specific signature genes (transcription factors and cytokines) after 3-d lineage-specific cell differentiation from mouse primary naïve CD4+ T cells in a dose-dependent manner.