Fig. 5.

RPS3 Ssu⁻ alleles reduce bulk translation initiation. (A–F) Strains from Fig. 2A were cultured in SC-Leu at 30 °C to an A₆₀₀ of ∼1.0, and cycloheximide was added (50 μg/mL) before harvesting. WCEs were separated by sucrose density gradient centrifugation and scanned at 254 nm. Mean (±SEM) polysomes:monosomes (P/M) and free 40S:60S ratios from three biological replicates are indicated. Asterisks indicate significant differences between mutant and WT as judged by a two-tailed, unpaired Student’s t test (*P < 0.05; **P < 0.01).