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. 2017 Feb 27;114(11):2904–2909. doi: 10.1073/pnas.1620799114

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Fig. 6. — One molecule of 18:0/20:4 PC binds specifically to the Na,K-ATPase. (A) Mass spectra of WT Na,K-ATPase in the presence of 0, 10, and 40 µg 18:0/20:4 PC, 0.6 mg/mL UDM, and 0.03 mg/mL 18:0/18:1 PS. (B) Hill-fit of peak intensity as a function of the 18:0/20:4 PC concentration (n_Hill = 1.05). Normalization was done by calculating the ratio of α₁β₁•2lipids/α₁β₁ for all added PC concentrations. The maximum ratio then was normalized to 1. Error bars represent the SEM. n = 3. (C) Mass spectrum of Na,K-ATPase in the presence of 30 µg/mL 18:0/18:1 PC showing no binding of the 18:0/18:1PC.