Fig. 5.

vps4Δ NEs have persistent fenestrations, karmellae, and disorganized extensions, and these defects are suppressed by loss of cmp7 or lem2. (A) WT cells with normal NE. (B–D) vps4Δ cells with a variety of NE phenotypes, including karmellae (B), multiple holes (arrows) (C), and discontinuities (black arrowhead) that can contain apparently intranuclear NPCs (D, arrowheads). (E–H) cmp7Δ and lem2Δ single mutants (E and G) vs. vps4Δcmp7Δ and vps4Δlem2Δ double mutants (F and H). (Scale bars: 500 nm.)