Fig. S5.

A cartoon model for the NE phenotypes in vps4Δ, lem2Δ, and cmp7Δ in S. pombe. (Top) In WT, the exposed Lem2p recruits Cmp7p to initiate an ESCRT-III–mediated NE sealing event. That process is regulated and/or completed by the AAA ATPase, Vps4p. (Middle) in vps4Δ, unrestricted Lem2p/Cmp7p activities induce the formation of karmellae and large gaps in NE so that the proper progress of mitosis is disrupted. (Bottom) In lem2Δ or cmp7Δ, mutant cells tolerate a limited number of small breaches and therefore show little or no growth defect.