Fig. 7.

RIP3 inhibitor GSK’872 blocks human platelet aggregation and in vivo thrombus formation. (A and B) Washed human platelets were incubated with different concentrations of GSK′872 or vehicle (DMSO) at 37 °C for 30 min, and then stimulated with U46619 (A) and thrombin (B). Platelet aggregation was monitored using a turbidimetric aggregometer. The traces are representative of 3 independent experiments. (C) Representative images of FeCl₃-induced mesenteric arteriole thrombosis in C57d mice injected with GSK′872 or vehicle (DMSO) as recorded by real-time microscopy. Time after FeCl₃-induced injury is indicated at the bottom right of each image. (D) The occlusion time of mesenteric arteriole injured by FeCl₃ in C57d mice injected with GSK′872 or vehicle. Data are mean ± SEM values of 15 controls and 11 GSK′872-injected mice. **P < 0.01. (E) RIP3, interacting with Gα₁₃, regulates integrin outside-in signaling.