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PF4 release from WT and RIP3−/− mouse platelets stimulated with U46619 and thrombin. Washed WT and RIP3−/− mouse platelets were stimulated with different concentrations of U46619 (A) or thrombin (B) for 8 min at 37 °C with stirring. The platelet suspensions were centrifuged, and the supernatants were analyzed for PF4 release using the PF4 ELISA Kit (Abcam). Quantitative results are expressed as mean ± SEM of three independent experiments.
