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PS exposure in mouse platelets stimulated with thrombin and U46619. Washed WT and RIP3−/− platelets were stimulated with different concentrations of thrombin (A) or U46619 (B), or with thrombin (1 U/mL) + collagen (5 μg/mL) (C) for 30 min at 37 °C. The platelets were further incubated with Annexin V-FITC for 10 min and then analyzed by flow cytometry. Results from a representative experiment stimulated with selected concentrations of thrombin and U46619 are shown.
