Fig. S11.
The interaction of Gα13 with RIP3. Increasing concentrations of Gα13 (His-tag; NewEast Biosciences) were incubated with immobilized RIP3 (Abcam) or BSA (control). The binding of Gα13 was assessed with mouse anti-His antibody and HRP-conjugated goat anti-mouse antibody. The absorbance at 450 nm was measured in four independent experiments, and values are presented as the mean ± SD after subtracting the binding of Gα13 to BSA (background). After the background signal had been subtracted, the binding curve was fitted to the equation Y = Bmax × x/(Kd + x), where Y is the specific binding, x is the ligand concentration, Bmax is the binding maximum, and Kd is the equilibrium dissociation constant. For some data points, the error bars are smaller than the symbols.