Fig. S14.

RGDS peptides abolish U46619-induced Akt phosphorylation. Washed WT and RIP3^−/− mouse platelets were preincubated with RGDS (2 mM) or vehicle (−RGDS) at 37 °C for 5 min. Then 350 nM U46619 was added to platelets in an aggregometer with stirring for 5 min. The platelets were lysed and analyzed by Western blot analysis with anti–phospho-Akt (Thr³⁰⁸) and anti-total Akt antibodies. The Western blot shown is representative of at least three independent experiments.