Fig. S2.

Impact of prolonged expansion of SON transduced NPC in GEM on oligodendroglial differentiation efficiencies and yields. SON-transduced NPC were expanded in GEM for either 4 (blue), 8 (green), or 12 d (red) and subsequently transferred to DM for an additional 28 d. FACS analyses at days 0, 14, and 28 of differentiation revealed a trend to decreased differentiation efficiency after 12 d of expansion (red curve) (A), whereas yields of O4⁺ cells (red columns) were significantly increased (B). Data are presented as mean of replicates from three independent experiments, each using an independent iPSC-derived NPC line + SD. Two-way ANOVA with Tukey’s multiple comparisons test was performed for statistical analysis (**P < 0.01, ***P < 0.001).