Fig. 3.

Strand photodissociation power dependence and kinetic scheme. (A) Overall photodissociation rate constant k_off vs. incident light power corrected for sample volume (P₀/V; reported as moles of photons per second per liter) for s7:loop:GFP, s10:loop:GFP (203T), s10:loop:YFP (203Y) (SI Appendix, Fig. S7C), and s10:loop:GFP (209Q) (discussed later), all with E222 retained and irradiated with 488-nm laser at 21.9 °C. Data points are fit well with Eq. 1a. Parameters are listed in SI Appendix, Table S5. (B) Kinetic scheme for the photodissociation process; φ_fwd and φ_rev are the forward and reverse quantum yields, respectively, and k_th and k_diss are the thermal relaxation and dissociation rate constants, respectively.