Fig. 5.
s10:loop:GFP E222Q strand photodissociation power dependence. (A) UV-vis spectra at representative time points for strand swapping of 3 μM s10:loop:GFP E222Q mixed with 90 μM s10203Y and continuously irradiated with 1.6 mW/mL 488-nm laser at 21.9 °C. Spectral deconvolution of intermediate time points is shown in SI Appendix, Fig. S14. (B) Population kinetics extracted from A. Trans GFP follows double-exponential kinetics. (C) The product and (D) the sum of k+ and k− as functions of P0/V, both including linear fits.