Table S1.
Data collection and refinement statistics
| Value | ||||
| Property | IFIT1 (L457E/L464E) m7Gppp-AAAA | IFIT1 (L457E/L464E) Gppp-AAAA | IFIT1 (L457E/L464E) PPP-AAAA | IFIT1 (N216A/L457E/L464E) m7Gppp-AAAA |
| Data collection | ||||
| PDB ID code | 5UDI | 5UDJ | 5UDK | 5UDL |
| Space group | P4222 | P4222 | P4222 | P4222 |
| Molecule per ASU | 1 | 1 | 1 | 1 |
| Cell dimensions | ||||
| a, b, c (Å) | 111.7, 111.7, 93.2 | 112.5, 112.5, 93.5 | 112.4, 112.4, 93.2 | 111.8, 111.8, 93.0 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 |
| Resolution (Å) | 50–1.58 (1.61–1.58) | 50–1.69 (1.72–1.69) | 50–1.65 (1.68–1.65) | 50–1.65 (1.68–1.65) |
| Completeness (%) | 100 (100) | 100 (100) | 99.7 (95.2) | 100 (99.9) |
| Rpim (%) | 2.4 (43.8) | 4.1 (52.6) | 2.4 (36.8) | 2.8 (39.6) |
| <I/σ(I)> | 33.6 (1.74) | 22.2 (1.9) | 30.3 (1.96) | 27.4 (1.78) |
| Redundancy | 14.5 (12.4) | 14.5 (12.2) | 14.2 (9.4) | 14.6 (12.3) |
| Refinement | ||||
| Resolution (Å) | 40.1–1.58 (1.6–1.58) | 40.3–1.69 (1.71–1.69) | 44.3–1.65 (1.67–1.65) | 40.1–1.64 (1.67–1.64) |
| No. reflections | 81,063 (7,992) | 67,665 (6,640) | 71,917 (6,888) | 71,805 (6,876) |
| Rwork/Rfree | 15.7/17.8 (25.0/27.6) | 15.9/18.1 (24.8/30.3) | 16.4/18.4 (24.2/26.8) | 16.4/18.2 (24.3/24.6) |
| No. of non-H atoms | ||||
| Protein | 3,842 | 3,852 | 3,833 | 3,816 |
| Ligand - RNA | 168 | 116 | 97 | 117 |
| Ligand - PEG | 10 | 10 | 41 | 10 |
| Ligand - ion | 2 | 2 | 2 | 2 |
| Solvent | 394 | 382 | 335 | 313 |
| Average B factors (Å2) | ||||
| All atoms | 30.65 | 27.64 | 30.61 | 30.86 |
| Protein | 30.7 | 27.6 | 30.4 | 30.9 |
| Ligand–RNA | 18.8 | 17.0 | 19.3 | 22.2 |
| Ligand–PEG | 49.2 | 48.5 | 57.4 | 62.6 |
| Ligand–ion | 32.1 | 30.8 | 26.6 | 39.5 |
| Solvent | 34.8 | 30.8 | 32.6 | 32.6 |
| Rms deviations | ||||
| Bond lengths (Å) | 0.017 | 0.014 | 0.012 | 0.008 |
| Bond angles (°) | 1.579 | 1.283 | 1.166 | 1.005 |
The highest-resolution shell is in parenthesis.